Using deep learning for predicting the dynamic evolution of breast cancer migration

Garcia-Moreno, F.M.  Ruiz-Espigares, J.  Gutiérrez-Naranjo, M.A.  Marchal, J.A.

Revista: Computers in Biology and Medicine

ISSN: 1879-0534, 0010-4825

Año de publicación: 2024

Volumen: 180

DOI: 10.1016/J.COMPBIOMED.2024.108890

Resumen

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Background: Breast cancer (BC) remains a prevalent health concern, with metastasis as the main driver of mortality. A detailed understanding of metastatic processes, particularly cell migration, is fundamental to improve therapeutic strategies. The wound healing assay, a traditional two-dimensional (2D) model, offers insights into cell migration but presents scalability issues due to data scarcity, arising from its manual and labor-intensive nature.

Method: To overcome these limitations, this study introduces the Prediction Wound Progression Framework (PWPF), an innovative approach utilizing Deep Learning (DL) and artificial data generation. The PWPF comprises a DL model initially trained on artificial data that simulates wound healing in MCF-7 BC cell monolayers and spheres, which is subsequently fine-tuned on real-world data.

Results: Our results underscore the model’s effectiveness in analyzing and predicting cell migration dynamics within the wound healing context, thus enhancing the usability of 2D models. The PWPF significantly contributes to a better understanding of cell migration processes in BC and expands the possibilities for research into wound healing mechanisms.

Conclusions: These advancements in automated cell migration analysis hold the potential for more comprehensive and scalable studies in the future. Our dataset, models, and code are publicly available at https://github.com/frangam/wound-healing.